We have developed a two-step, real-time, quantitative assay, using the polymerase chain reaction with reverse transcription (RT-PCR) and based on SYBR Green I monitoring of PCR product accumulation, ...
Gene expression experiments measure changes in RNA transcript levels to compare how genes are up- or down-regulated across ...
Announcing a new article publication for Zoonoses journal. Lone Star virus (LSV) is a newly characterized arbovirus with pathogenic potential. However, no detection methods are available to ...
Announcing a new article publication for Zoonoses journal. Lone Star virus (LSV) is a newly characterized arbovirus with pathogenic potential. However, no detection methods are available to ...
Although 5'-3' nuclease oligoprobe (TaqMan assay) is the most widely used format owing to its commercial utility and higher specificity as a result of sequence-specific hybridization of the probe, the ...
Quantitative PCR (qPCR) technologies have exploded in the last decade. Commonly used for both diagnostics and basic research, new and exciting advances are being made in such areas as biomarker ...
Recent advances in PCR amplification methods, which hold a great deal of promise for highly specific and sensitive virus detection assays, combine PCR amplification with fluorescently-labeled ...